ABSTRACT
Objective To investigate the molecular mechanism of SNHG5 regulating the proliferation, invasion and apoptosis of glioblastoma multiforme (GBM) cells by targeting miR-421. Methods Real-time quantitative PCR test was performed to detect the expression levels of SNHG5 and miR-421 in 31 cases of GBM tissue samples and 32 cases of normal brain tissue samples. After increasing or decreasing SNHG5 expression in U87 cell lines by lentivirus or plasmid transfection, the changes of miR-421 expression were measured by real-time quantitative PCR, to explore the correlation between SNHG5 and miR-421 in GBM. The dual-luciferase reporter test was performed to explore the target interaction of SNHG5 and miR-421. The plasmids with low expression of SNHG5 and miR-421 were cotransfected into U87 cells for the rescue experiment. CCK-8 test, Transwell test, flow cytometry and tumor cell xenograft in nude mice were used to verify molecular mechanism of SNHG5 regulating the proliferation, invasion and apoptosis of GBM in vitro and vivo. Results The expression level of miR-421 was decreased in U87 cell line after SNHG5 upregulation. In addition, the expression level of miR-421 was increased in U87 cell line after SNHG5 downregulation (P < 0.05). The expression level of SNHG5 was correlated negatively with the expression of miR-421 in GBM and U87 cell line. The result of luciferase reporter tests indicated SNHG5 targetedly interacted with miR-421. Rescue experiment results showed that compared with si-SNHG5+miR-421-inhibitor group, the proliferation, invasion and anti-apoptosis ability of U87 cells were significantly inhibited in the si-SNHG5+control-inhibitor group, the expression levels of BAX and p21 were significantly higher, the expression levels of CyclinD1 and Bcl-2 were lower remarkably (P < 0.05). Conclusion SNHG5 promotes the proliferation, invasion and anti-apoptosis of GBM by targeting miR-421 and regulating the expression of CyclinD1, p21, BAX and Bcl-2. Downregulation of miR-421 is related to SNHG5 overexpression in GBM.
ABSTRACT
Objective To investigate the prevalence and influencing factors on respiratory disease related symptoms (coughing,expectoration,persistent coughing and persistent expectoration) in Chinese people ≥40 years old.Methods Data was collected from 162 surveillance points in the China Non-communicable and Chronic Disease Surveillance Project in 2010.A total number of 56 066 people,aged ≥40 years,were selected by multi-stage cluster random sampling method in this study.The prevalence rates of coughing,expectoration,persistent coughing and persistent expectoration were calculated according to gender by x2 test.Multivariate logistic regression model was used to analyze the related influencing factors.Results The prevalence rates of coughing,persistent coughing,expectoration and persistent expectoration in men were significantly higher than that in women (P＜ 0.01).The prevalence rate of respiratory symptoms was positively correlated with age and duration and amount of cigarette smoking (P＜0.01),while negatively correlated with participants' education level (P＜0.01).Results from multivariate logistic regression analysis showed that gender,age,education level,smoking-related parameters,areas of residency (in urban or rural areas),etc.were appeared as influencing factors on the prevalence of respiratory symptoms.Conclusion The symptoms of chronic respiratory diseases appeared prevalent in Chinese population aged ≥40 years.Factors as older age,living in the central/western regions and smoking-related parameters were identified as of higher risks.
ABSTRACT
Objective To investigate the prevalence and influencing factors on respiratory disease related symptoms (coughing,expectoration,persistent coughing and persistent expectoration) in Chinese people ≥40 years old.Methods Data was collected from 162 surveillance points in the China Non-communicable and Chronic Disease Surveillance Project in 2010.A total number of 56 066 people,aged ≥40 years,were selected by multi-stage cluster random sampling method in this study.The prevalence rates of coughing,expectoration,persistent coughing and persistent expectoration were calculated according to gender by x2 test.Multivariate logistic regression model was used to analyze the related influencing factors.Results The prevalence rates of coughing,persistent coughing,expectoration and persistent expectoration in men were significantly higher than that in women (P＜ 0.01).The prevalence rate of respiratory symptoms was positively correlated with age and duration and amount of cigarette smoking (P＜0.01),while negatively correlated with participants' education level (P＜0.01).Results from multivariate logistic regression analysis showed that gender,age,education level,smoking-related parameters,areas of residency (in urban or rural areas),etc.were appeared as influencing factors on the prevalence of respiratory symptoms.Conclusion The symptoms of chronic respiratory diseases appeared prevalent in Chinese population aged ≥40 years.Factors as older age,living in the central/western regions and smoking-related parameters were identified as of higher risks.